Effects of Biosynthesized Zinc and Copper Nanoparticles on Medicine Resistant Pathogenic Bacteria(Antibiotics)

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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction

In this study, the antimicrobial activity of biologically synthesized zinc (Zn) and copper (Cu) nanoparticles was investigated on gram-positive and gram-negative bacteria, pathogens, and resistant and common nosocomial infections.

Material & Methods

Intially, zinc oxide and copper nanoparticles were synthesized using Xanthomonas campestris and Pseudomonas stutzeri bacteria, respectively. To investigate the effect of different concentrations of nanoparticles on bacteria using the macrodilution method, concentrations of 0.01, 0.1, 0.5, 1, and 1.5% of Zn and Cu nanoparticles (culture medium + nanoparticles) were prepared and were added to the respective bacteria at a concentration of 105 cell/ml. Containers containing treated media (bacteria + nanoparticles) and control media were placed in a shaker incubator. Afterward, the optical density (OD) of treatment and positive control and negative control media were determined.

Findings

The results of statistical analysis showed that Zn and Cu nanoparticles, at a concentration of 0.5%, were able to remove almost all (100%) Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus bacteria and were bacteriostatic at the concentration of 0.1%.

Discussion & Conclusion

The results obtained from the determination of antibacterial properties of nanoparticles showed a direct relationship between the concentration of nanoparticles and the percentage of bacterial removal.

Language:
English
Published:
Ilam University of Medical Science, Volume:30 Issue: 5, 2022
Pages:
40 to 50
https://www.magiran.com/p2515688  
سامانه نویسندگان
  • Nabipour، Yasaman Sadat
    Author (1)
    Nabipour, Yasaman Sadat
    (1395) دکتری میکروبیولوژی، دانشگاه ایلام
  • Ahmady، Salman
    Author (5)
    Ahmady, Salman
    Associate Professor Microbiology, University of Mazandaran, بابلسر, Iran
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