Interfering Effects of ethanolic extract of Propolis, Thymus vulgaris essential oil and Silver Nanoparticles on bacteria and Candida albicans in vitroو observation

Thymus vulgaris and silver Nanoparticles has been demonstrated. Each of these agents for maximum impact requires a high concentration of substances affecting microorganisms. Assuming an anti-microbial agent with low concentration and in combination with other anti-microbial agents as the initial impact or more, A study to evaluate the effect of ethanol extract of propolis interference, Thymus vulgaris essential oil and silver nanoparticles on gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae, Listeria monocytogenes) and Gram-negative bacteria (Proteus mirabilis, Klebsiella pneumonia and Pseudomonas aeruginosa) and on Candida albicans was performed. Microbial used in this study are taken from Iranian Research Organization for Science and Technology. Propolis is collected from different area of west Azerbaijan beehives. The ethanolic extract of propolis have prepared after 3 days. The interfering effects of these agents are evaluated by Micro-broth dilution method on Muller Hinton Broth in the presence of gentamicin as positive control for bacteria and Nistatin against Candida albicans. Acquired Minimum Inhibitory Concentration (MIC) show the effective interfering of these agents. The results of the Minimum Inhibitory Concentration (MIC) of propolis extract with silver nano particle on Klebsiella pneumonia was 25 µgr/ml, on Streptococcus pneumonia, Pseudomonas aeruginosa and Candida albicans was 50µgr/ml and on Staphylococcus aureus, Listeria monocytogenes and Proteus mirabilis was 100 micrograms per milliliter. MIC of Thymus essential oil with silver nanoparticles on Klebsiella pneumonia and Pseudomonas aeruginosa was 50µgr/ml, on Staphylococcus aureus, Streptococcus pneumonia and Candida albicans was 100 µgr/ml and on Listeria monocytogenes and proteus mirabilis was 200 micrograms per milliliter.