Effect of using enzyme and Lactic acid bacterial inoculant on digestibility, protozoa population and Methane production(CH4) of tomato pomace and pumpkin waste silage

This study was conducted to evaluate the effects of enzymes and lactic acid bacterial inoculant on the digestibility, methane production and protozoa population silage mix of tomato pomace and pumpkin waste. Representative of samples were packed manually, in triplicate into plastic bags and were stored at ambient temperature and allowed to ensile for 90 days. The following treatments were applied to the forage samples: 1) tomato pomace and pumpkin waste silage mix (50:50), without any additives (control), 2) control + LAB made inoculant (8×109 CFU/ml), 3) control + fibrolytic enzyme and 4) control + Fibrolytic Enzyme+ LAB made inoculants. The results showed partitioning factor between experimental treatments showed significant difference (P<0.035), that the most parsing factor was related to enzyme additive treatment. No significant difference was seen between treatments in terms of microbial mass production and gas production efficiency (P>0.05). But, microbial protein production efficiency was significantly different (P<0.05). In terms of protozoa population, there was a significant difference between experimental treatments of total population and halotric (P<0.05). But, in the population of Antonium, Diplodinium, Africavasculus, the difference was not significant (P>0.05). Methane production in total gaseous production and MRP was significantly different (P<0.05). But, there was no significant difference in methane (P>0.05). Overall, the results showed that the use of various additives had no significant effect on the nutritional value of the silage of the mixture of tomato pomace and pumpkin waste compared to the control treatment.