Studying the expression of ADGRL4 and ADGRF5 receptors in endothelial cells in hyperglycemic conditions under the influence of Atorvastatin

The endothelium layer plays an important role in maintaining vascular homeostasis. The activation and dysfunction of endothelial cells is one of  the first detectable changes in atherosclerosis as one of the macro-vascular complications of diabetes. In this study, according to the physiological function of G-protein coupled receptors in the vascular system, the changes in gene expression of two receptors, ADGRF5 and ADGRL4 were investigated in hyperglycemic conditions and the use of atorvastatin.

Endothelial cells were transferred from normal conditions to hyperglycemic environment and kept in these conditions for 48, 24 and 72 hours. In hyperglycemic conditions, the cells were treated with atorvastatin (5 and 10 µM) for 24 hours. The expression of target genes was measured in hyperglycemic conditions and under atorvastatin treatment, scratch test cell culture was performed in the presence of atorvastatin drug.

ADGRF5 and ADGRL4 gene expression decreased significantly p ≤ 0.01 in hyperglycemic environment at 24, 48 and 72 hours, the level decreased by about 7 to 9.5 times and about 7 to 28 times respectively for genes. Treatment with atorvastatin in hyperglycemic conditions resulted in a significant increase in the expression of receptors p ≤ 0.01. VEGFA and DLL4 showed a significant decrease in expression compared to hyperglycemic conditions. An increase in the power of cell movement and changes in the morphology of cells were observed when using atorvastatin.

Changes in the expression of ADGRF5 and ADGRL4 receptors in hyperglycemic conditions can focus on the role of these receptors in the process of angiogenesis in vascular disorders, which is useful for future studies related to vascular disorders related to diabetes complications.
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