Comparison of Common Available Methods in Identification of Ruminant's Blood Protozoa, Sistan Region of Iran

Theileriosis and Anaplasmosis are very prevalent in native ruminants of Sistan region of Iran. Bovine babesiosis could be found with fewer prevalence. Also, Trypanosoma evansi induce the Surra disease in the camels of the region. The objective of present study is study and comparison of common available methods in identification of such infective organisms including clinical appearance, giemsa staining and molecular techniques (2013-2018). The blood specimens of 636 cattle, 80 sheep and 213 camels have been investigated with giemsa staining and molecular methods. Also, the clinical features were evaluated. The current results indicated that molecular PCR amplification is a more sensitive tool as compared to smear scanning for the detection of blood protozoa. However, it doesn’t mean that molecular techniques could be considered more preferable. The advantages and disadvantages of each identification method is discussed comprehensively based on the type of the parasites, hosts and economical condition in the area.