Investigating of Histological and Pathological Effects of 4chloro-N (2-(4-methoxyphenil)-4-oxotiazolidin-3-yl)-2-phenoxybenzamid on Trichogaster trichopterus As Anticonvulsant

Effective anticonvulsant drugs each selectively suppress the abnormal activity of neurons to some degree. However, their mechanism of action and efficacy differ in different types of epilepsy disorders.

Investigation of histological effects of 4chloro-N (2-(4-methoxyphenil)-4-oxotiazolidin-3-yl)-2-phenoxybenzamid compared to Diazepam as a reference drug on Trichogaster trichopterus ‘s liver which has done at the fish lab of Islamic Azad University.

In this study, 150 fish specimens with an average weight of 2 ± 0.5 gr were bought from Khan-mahi fish farm. This study was performed on 4 treatment groups (1, 3, 5, 7 mg/kg b.w.) and three controls including, control 1 (intact), control 2 (solvent tween 80), reference control (Diazepam 3 mg/kg.b.w). Before dropping fishes into the aquariums, dechlorination of water was performed in 24 hours, and physicochemical factors were measured. Injections were performed every other day for 20 days and all the treatments were anatomized and their livers put into the fixative to check by optical and electron microscope later.

The results showed that the ALT, AST and ALP were changed in controls to treatments. These results showed a significant difference between the control and the treatment groups (P≤0.05) Microscopic images also showed membrane changes, rupture, gaps between sinusoids, and necrosis at a dose of 5 mg /kg body weight and was in a dose-dependent.