The Effect of Bud Position and Growth Regulators on the Micropropagation of Rosa damascena Mill.

Several experiments were conducted to study the effects of bud position and plant growth regulators on the micropropagation of Rosa damascena. In the first experiment, the effects of bud localities (the proximal, middle, distal and shoot tip), BAP (2.5 and 5 mgL-1), TDZ (0.05 and 0.1mgL-1) and NAA (0.1 mgL-1) were studied on the regeneration of plant. The highest shoot length in the distal, middle and proximal bud locations was attained by 2.5 and 5 mgL-1 BAP + 0.1 mgL-1 NAA. For the proximal and shoot tips, the top data was related to 0.05 mgL-1 of TDZ + 0.1 mgL-1 NAA. In the second experiment, Kin and BAP (0, 1.5, 3 and 4.5 mgL-1) treatment combinations were tested on the regeneration potential without considering bud location. The highest shoot length belonged to 4.5 mgL-1 of BA and BAP. For the rooting stage; 0.1, 0.2 and 2 mgL-1 of NAA and IBA were examined and the highest rooting percentage and root length was recorded for 0.1 mgL-1 IBA. In conclusion, the in vitro regeneration response was controversy for the bud location. However, the growth regulators had promising effects on the regeneration of Rosa damascena.