Evaluation of production performance, morphological and microbiological traits of broilers ileum of fed with the organic form of the zinc, copper, manganese and iron under heat stress

In this experiment, the effect organic forms of zinc, copper, manganese and iron on growth performance, morphology and microbial population of small intestinal using 360 one-day-old Ross 308 male broilers in a Completely randomized design with 6 groups and 6 replicates from 1 to 42 days of age under heat stress conditions. The experimental groups were: 1- basal diet (control), 2- basal diet + 5mg copper-methionine, 20mg manganese-methionine and 5mg iron-methionine (CuMnFe) 3-basal diet + 40mg zinc-methionine, 20mg manganese-methionine, 5 mg iron-methionine (ZnMnFe) 4-basal diet + 40mg zinc-methionine, 5mg copper-methionine, 5mg iron- Methionine (ZnCuFe) 5-basal diet +40mg zinc-methionin, 5mg copper-methionine, 20mg manganese-methionine (ZnCuMn) and 6-basal diet +40mg zinc-methionine, 5mg copper-methionine, 20mg manganese-methionine and 5mg iron-methionine (ZnCuMnFe). The consumption of CuMnFe and ZnCuMn groups increased the villus height as compared to other groups. The birds of all groups had the lower abdominal fat weight, higher muscle layer thickness and lower coliform population in ileum compared to control birds. The number of Lactobacillus bacteria in ZnMnFe and ZnCuMn birds was higher than that of the birds in other groups. In conclusion, consumption of ZnCuFe group in broiler diet under heat stress condition causes the decreased harmful microbial population in intestine and improved performance and breast and thigh weights.