Evaluation of the effect of Satureja sahendica hydroalcoholic extract and Trisodium Phosphate on biofilm formation of Salmonella Typhimurium isolated from poultry

Salmonella Typhimurium (S. Typhimurium) is one of the most important worldwide public health concerns capable of forming biofilms on food contact surfaces. The aim of this study was to evaluate the antibacterial and anti-biofilm activity of Satureja sahendica hydroalcoholic extract (SSHE) and Trisodium Phosphate (TSP) against S. Typhimurium (n= 20) isolated from poultry. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using the microdilution broth method. To evaluate the anti-biofilm effects, the isolates were treated with inhibitory and sub-inhibitory concentrations of both substances and after 24 hours, the biofilm formation was evaluated. According to the results, the MICs of SSHE and TSP against S. Typhimurium isolates were ranged from 1.25-10 and 5-80 mg ml-1, respectively. Also, MBCs value of SSHE and TSP were varied against isolates and were ranging from 40-160> and 80-160> mg ml-1, respectively. The results of statistical analysis showed that both of SSHE and TSP significantly reduced biofilm formation in S. Typhimurium compared to the control group (p <0.05). Also, the statistical results showed that SSEH reduces the rate of biofilm formation more than TSP (p <0.05). According to these results, SSHE can be a suitable alternative to chemicals such as TSP in the food industry to reduce bacterial contamination. However, its application requires further studies to determine its safety and toxicity.