The effect of 8 weeks of endurance-resistance training with taurine supplementation on the serum level of osteopontin and osteocalcin in Wistar diabetic male rats treated with streptozocin

There is a relationship between osteopontin and osteocalcin bone indices and diabetes and osteoporosis. The aim of this study was to determine the effect of 8 weeks of endurance training with taurine supplementation on the serum level of osteopontin and osteocalcin in male Wistar rats with streptozocin. In this experimental study, 30 adult non-nursing rats with a weight range of 250-300 grams and 6 weeks old were randomly divided into 3 groups including two diabetic groups, diabetic exercise+supplement (n=10) and diabetic control (n=10) and a non-diabetic group, healthy control (n=10). To induce diabetes, at the end of the eighth week, the amount of 55 mg per kilogram of body weight of streptozocin (STZ) was injected intraperitoneally. The exercise+diabetic supplement group performed combined exercises for eight weeks with a frequency of 5 times a week (endurance exercise with an intensity of 75% Vo2max on the treadmill and then strength training, climbing the ladder 15 times). At the same time as the exercises started, taurine supplement was added to the rats' food. One-way ANOVA and post hoc Bonferroni test were used to compare the mean of the variables between-group and paired samples T test was used to compare the mean of the variables intra-group. Osteopontin decreased significantly in the diabetic exercise+supplement group compared to the diabetic control group (P<0.001); On the other hand, there was a significant increase of 12.118% in the diabetic control group compared to the healthy control group (P<0.001). While, osteocalcin increased significantly in the exercise+diabetic supplement group compared to the diabetic control group (P<0.001) and decreased significantly in the diabetic control group compared to the healthy control group (p<0.001). Eight weeks of endurance training with taurine supplementation led to a decrease in osteopontin and an increase in osteocalcin. Therefore, people with diabetes and prone to osteoporosis are recommended to do combined exercises to prevent the development of this diseases.