The effect of six weeks of aerobic exercise and melatonin on MBP gene expression and pain threshold in an experimental model of diabetic neuropathic pain

Diabetic peripheral neuropathy is one of the most common chronic complications of diabetes. The aim of this study was the effect of six weeks of aerobic training and melatonin on MBP gene expression and pain threshold in an experimental model of diabetic neuropathic pain.

Forty 8-week-old male Wistar rats (weight range 204 ± 11.3 g) were randomly divided into five groups (n=8 each) including: diabetic neuropathy (50 mg / kg streptozotocin intraperitoneal injection), diabetic melatonin neuropathy (mg / kg 10 melatonin daily for 6 weeks), diabetic neuropathy exercise (30 minutes of aerobic exercise at 15 meters per minute, 5 days a week for 6 weeks), diabetes melatonin neuropathy and healthy exercise and control. After confirmation of diabetic neuropathy by behavioral tests, exercise protocol and supplementation were performed. MBP gene expression in spinal cord tissue was measured by real-time technique.

Exercise and melatonin reduced the nervous system's sensitivity to thermal hyperalgesia and mechanical allodynia (P=0.026). Aerobic exercise with melatonin significantly increased MBP gene expression compared to diabetic neuropathy group (P =0.001).

Aerobic exercise and melatonin appear to reduce the complications of diabetic neuropathy by increasing MBP protein. It is recommended to use aerobic exercise with melatonin for diabetics to reduce the neuropathic pain of diabetes.