Evaluation of serum complement activity in Mugger crocodiles (Crocodylus palustris)

Little is known about complement system as a component of innate immunity of the ectothermic vertebrates such as crocodiles. Serum complement is valuable tool in determining the health status of turtles. In this research effect of concentration, volume and temperature on alternative complement pathway activity of Mugger crocodiles (Crocodylus palustris) using standard haemolytic assays was studied. For this purpose, Crocodylus palustris serum complement hemolysis in different concentrations, volumes and temperatures was measured. Results indicated significant relation between relation measured hemolysis and concentration, volume and temperature (p<0.05). The measure of hemolysis in various concentrations (25%, 50% and 100%), volumes (10, 20, 30 µL) and temperatures (5-35°C) were significantly increased (p<0.05). The results show that alternative complement pathway activity of crocodiles might affect concentration, volume and temperature that it caused increased non-specific immunity and the resistance to the outbreak of diseases.