Investigation of antimicrobial properties of green synthesized CuO nanostuctures using Berberis integerrima aqueous extract on infected surgical wound caused by Staphylococcus aureus

Staphylococcus aureus bacteria causes a wide range of infectious diseases. In this study, the antimicrobial activity of the synthesized copper oxide nanostructure in the presence of Berberis integerrima aqueous extract on the infected surgical wound caused by this bacterium was investigated in mice.

First, copper oxide nanostructures were synthesized in the presence of Berberis integerrima aqueous extract and confirmed by XRD, FT-IR, FE-SEM analyses, and the MIC and MBC levels of the nanostructures used were determined by the dilution method in Mueller Hinton Broth culture medium. Also, after creating a skin wound and inoculating the solution of Staphylococcus aureus bacteria with a concentration of (5 × 105 CFU/ml), the concentration of MBC along with Eucerin ointment was checked on the infected wounds.

In general, copper oxide nanostructure synthesized in the presence of Berberis integerrima aqueous extract has a significant antimicrobial activity to control the growth of Staphylococcus aureus bacteria. Also, the synthesized copper oxide nanostructure has a favorable antimicrobial and reparative effect on the infected surgical wound caused by this bacterium.