Investigating the effect of sweet paste containing AVP-4 اNano-supplement on queen laying, population size and biochemical characteristics of the carcass in honey bee (Apis mellifera) colonies.

The aim of this research is to investigate the effect of sweet paste containing AVP-4 on queen egg laying rate, carcass weight, population size, brood rearing, protein and body fat in European honey bees. In this experiment, 20 colonies with the same age and homogenized queen were used. The hives used were of the Langstroth type and the colonies were homogenized in terms of the population of newborns, the population of adult bees, and the amount of food reserves (pollen and honey). The field experiment was carried out in the form of a block design in four treatments, including the first control treatment of sweet dough without Nano-supplement, the second treatment of sweet dough containing 2.5% , the third treatment of sweet dough containing 5% , and the fourth treatment of sweet dough containing 7.5% of Nano-supplement in five replications. The amount of eggs, larvae and pupae was measured every 15 days and the colony population was measured in a frame. It was used to determine crude protein and body fat based on AOAC methods. The obtained results showed that there was no significant difference between the treatments in the entire experimental period by comparing the mean egg laying of the queen, brood rearing, population size and crude protein in the entire experimental period (P>0.05). Comparison of the average amount of fat (ether extract) had a significant difference among the treatments (P<0.05). The use of AVP-4 supplement in feeding honey bees had no significant difference between the treatments .