Evaluation of Antioxidant, Anti-inflammatory and Antibacterial P with Anbernesara Smoke Extract

The aim of this study is to synthesize and investigate the anti-oxidant, anti-inflammatory and anti-bacterial effects of polylactic acid (PLGA) nanoparticles containing the extract of Anbernesara smoke. For this purpose, Anbernesara smoke was extracted and encapsulated by PLGA nanoparticles using the W1/O/W2 method and characterized by DLS, zeta potential, FESEM and FTIR electron microscope techniques. The antioxidant activity of the synthesized nanoparticles using ABTS and FRAP tests, as well as measuring the expression levels of the antioxidant genes catalase (CAT) and superoxide dismutase (SOD) and the expression of interleukin 6 (IL-6) and interleukin 10 (IL- 10) was also analyzed to determine the anti-inflammatory potential of nanoparticles by Real time PCR test. The antibacterial activity of the synthesized nanoparticles was evaluated on two bacterial strains Escherichia coli and Bacillus subtilis. The results showed the formation of spherical nanoparticles with an average size of 51.2 nm, dispersion index of 0.22, surface’s charge of -35.37 mV and encapsulation efficiency of 74%. The antioxidant properties of synthesized PLGA nanoparticles were confirmed by reducing Fe+3, inhibiting the formation of free radicals and increasing the expression level of CAT and SOD genes. The expression levels of IL-6 and IL-10 genes were also increased under the influence of PLGA nanoparticles loaded with the organic extract of Anbernesara smoke, indicating the anti-inflammatory properties of the proposed nanoparticles. PLGA nanoparticles loaded with the organic extract of Anbernesara smoke had an antibacterial effect on B. subtilis, but not on E. coli. The PLGA nanoparticles loaded with the organic extract of Anbernesara smoke have anti-oxidant, anti-inflammatory properties and can be considered as a combination with antibacterial potential.
.