Evaluation of polycaprolactone (PCL) based nanogel containing tetracycline on experimental wound infected with staphylococcus aureus bacteria healing on rat’s skin

The aim of this study was to evaluate the histopathology of nanogel containing tetracycline on experimental wounds infected with staphylococcus aureus on the skin of rats. This study used eight adult male Wistar rats (12-14 weeks old) weighing 250-300 gr. These rats were transferred to the Faculty of Veterinary Medicine's animal rearing center laboratory and kept in standard cages under standard conditions. Anesthesia was induced by intraperitoneal injection of 2% xylazine hydrochloride (10 mg/kg / IP) and 10% ketamine hydrochloride (60 mg/kg / IP). After general anesthesia, the rats were placed abdominally on the surgical table, then the dorsal surface of the rats was prepared and scraped from the scapula to the ileum, and two circular wounds of 7 mm in diameter were 1 cm apart and 1 cm apart. 1.5 cm from each other was created using a 7 mm sterile biopsy punch. Epidermal layers were obliterated by incision. The wound surface of all rats was infected with 1 ml of suspension containing 108 CFU / ml of Staphylococcus aureus, which was created by the 0.5 McFarland method. The rats were assigned into two equal groups (n=4 each) as follows: control group with no medication and receiving nanogel containing tetracycline. At the end of days 3, 7, 14, and 21, specimens were obtained for histopathological examination by scalpel.‏ Based on the results, nano gel containing tetracycline has excellent and acceptable effects on the healing process and wound healing in rats.