The relationship between rs896854 polymorphism of TP53INP1 gene and type 2 diabetes in Delijan City, Iran

Genetic engineering studies on the TP53INP1 gene have determined that a dysfunctional mitochondrial mitophagy disorder is the main source of chronic oxidative stress and leads to an increased susceptibility to diabetes. The aim of this study was to evaluate the relationship between rs896854 polymorphism of TP53INP1 gene and type 2 diabetes.

In this case-control study, blood samples were collected from 124 patients with type 2 diabetes and 100 healthy individuals from the residents of Delijan City, Markazi Province, Iran, in 2022. After extracting genomic DNA by the salting out method, the rs896854 location in the TP53INP1 gene was evaluated using the Tetra-Primer Arms-PCR method.

The frequency rates of TT, CC, and TC genotypes were 13%, 29%, and 58% in the patient group and 12%, 17%, and 71% in the control group, respectively. In addition, the frequency rates of T and C alleles were 42% and 58% in the patient group and 47.5% and 52.5% in the control group, respectively. Statistical analysis using the Chi-square test revealed that the rs896854 polymorphism of TP53INP1 gene had no significant relationship with the occurrence of diabetes in the studied population (P<0.05).

According to the current findings, the presence of T or C allele at rs896854 location in TP53INP1 gene had no significant relationship with the probability of the occurrence of type 2 diabetes. To confirm these findings, it is necessary to carry out further investigations in different populations with a larger sample size.