Evaluation of the effect of Mentha piperata and Mentha Pulegium extract on blood and biochemical parameters in Oncorhynchus mykiss

In recent years, the application of herbal compounds has replaced artificial supplements in the fisheries due to their benefits. This study was performed to investigate the effects of Mentha piperata and Mentha Pulegium extracts on some blood and biochemical parameters in Oncorhynchus mykiss. For this purpose, 320 fish were evaluated in a completely randomized design with six treatments and four replications in a farm around Kermanshah Province. Experimental groups included (1): control group (basic diet), (2): treatment containing 1% Pennyrile, (3): treatment containing 2% M. Pulegium, (4): treatment containing 1% M. piperata, (5) treatments contained 2% M. piperata and (6) combination treatments (1% of each extract). Blood parameters including white and red blood cells, globulin, hemoglobin, and biochemical parameters, including albumin, glucose, triglycerides, and the enzymes alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase were evaluated. According to the results, the combination of M. pulegium and M. piperata extracts resulted in more white blood cells, red blood cells, and hemoglobin compared to the individual levels of both mint extracts. 1% M. piperata extract had the greatest effect on blood globulin count. The most significant effect on albumin content was observed in 2% M. piperata extract group. The highest mean blood glucose and albumin were found in the fish received 2 percent of M. piperata. Also, the highest levels of and the lowest triglyceride were observed in the fish supplemented with M. piperata extract and the combination of M. piperata and M. pulegium extracts, respectively. Higher levels of both M. pulegium and M. piperita L extracts increased the levels of alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase enzymes. According to the results of the study, it seems that in physiological conditions, the use of M. pulegium and M. piperata extracts can improve the physiology of fish while improving the function of the immune system and some blood biochemical parameters.