Comparison of the effect of 6-week aerobic and high intensity interval training on expression of uPA and uPAR in the tumor tissue of female mice with breast cancer

In the past few decades, research on the role of physical activity in the prevention of cancer and disease progression has been the subject of attention of many researchers. The aim of this study was to compare the effect of 6 weeks of aerobic training and high intensity interval training on the expression of uPA and uPAR in the tumor tissue of female mice with breast cancer.
24 female Balb c mice with breast cancer were randomly divided into 3 groups: tumor (T), tumor + aerobic training (T+AE) and tumor + high intensity interval training (T+HIIT). The T+AE group had incremental endurance treadmill running for 6 weeks/5 days/60 minutes. The T+HIIT group had a 6-week/3-day/60 min high intensity interval training protocol. Then, the mice were killed, the tumor tissue was removed and frozen in liquid nitrogen. The expression of uPA and uPAR genes was measured by Real Time-PCR method. ΔCT, ΔΔCT, fold change, one-way ANOVA and Tukey's tests were performed at a significant level P<0.05 with GENEX software .
The results of the present study showed that in the T+AE group compared to the T group, the expression of uPA and uPAR decreased (P=0.00) 2.07 and 2.55 times. In the T+HIIT group compared to the T group, the expression of uPA and uPAR decreased 4.25 and 7.74 times (P=0.00), and in the T+HIIT group compared to the T+AE group, the expression of uPA and uPAR decreased 2.04 and 3.03 times (P=0.00).
Aerobic training and high intensity interval training are probably effective in preventing the progression of breast cancer by reducing the expression of uPA and uPAR.