The effect of three types of exercise training on the expression of 1β-TGF and WASH-1 genes in the left ventricle of male Wistar rats.

Regular sports activity is an effective non-pharmacological method in the prevention of diseases, and determining the type of activity, its intensity and duration as a suitable model helps to prevent cardiovascular diseases. The purpose of this research was to investigate the effect of three training methods on TGFB1 and VASH-1 gene expression in the left ventricular tissue of Wistar rats.

The research method was an experimental type with a post-test design with a control group. 40 8-week-old Wistar rats with an average weight of 237+33 grams were kept in a controlled environment and randomly divided into 5 groups of 8 each, the control group (CO), the control group for 8 weeks. (CO8), moderate-intensity aerobic exercise (MIT), high-intensity aerobic exercise (HIT), and high-intensity interval aerobic exercise (HIIT). Training programs were carried out for 8 weeks and 3 sessions per week with specific intensity. Aerobic exercises with moderate intensity included 47 minutes of running with an intensity of 65% VO2MAX on a treadmill. High-intensity training included running at a speed of 2 meters per minute with an increasing slope on the treadmill and high-intensity intermittent aerobic training including 4 intense intermittent bouts with an intensity of 90-100% VO2MAX and four low-intensity intermittent bouts of 50-60% VO2MAX. The basic control group underwent surgery and tissue removal in the first week, the other groups were dissected and heart tissue was removed after the last training session. Outgroup changes were analyzed by oneway analysis of variance and James Hoel's post hoc test at a significance level of P≥0.05.

The results show that 8 weeks of moderate intensity (MIT), high intensity (HIT) and high intensity interval (HIIT) training significantly increased the expression of TGF- β1 in the left ventricular tissue of Nervestar rats (P=0.001), but No significant difference was observed between the two control groups and the 8-week control group (P=1.000). Also, these exercises led to a significant decrease in VASH-1 gene expression in the research groups (P=0.0001, which is the most significant change It is related to HIT exercise

From the findings of this research, it can be concluded that regular exercises have the potential to affect cardiac hypertrophy by increasing TGF-β1 and decreasing VASH- 1 in male Wistar rats, and this effect is more under HIT exercises.