Relationship of rs6499640 and rs17817449 Polymorphisms in the FTO Gene with Type 2 Diabetes

Diabetes is a growing global health problem and caused by the interaction of genetic and environmental factors. One of the most important genes related to type 2 diabetes is the FTO gene. The aim of this study was to investigate the relationship between rs6499640 and rs17817449 polymorphisms in the FTO gene with type 2 diabetes in the population of Dezful city. In this research study, samples consist of 100 normal people and 100 people with type 2 diabetes were included in the study. DNA extraction was performed after blood sampling. The quantity and quality of extracted DNA was measured using nanodrop and electrophoresis. ARMS-PCR and Tetra-ARMS-PCR technique was used to determine the genotype of samples. Then was used sequencing for confirming of the genotyping results by PCR and data were analyzed statistically. There was no significant relationship in the distribution of genotypes between rs6499640 and rs17817449 polymorphisms of the FTO gene and the increased risk of diabetes in the population of Dezful city was not shown (P value equal to 0.728 and 0.085, respectively). But in the case of rs17817449 polymorphism, a significant relationship was found between the frequency of GG genotype and the risk of type 2 diabetes. According to this study results, association between current studied polymorphisms and causing of type 2 diabetes in the population of Dezful city was not declared. For confirming of these results, more studies in the larger population and more sampling are recommended.