Expression of MHC Class I and ICAM-I Association between Breast Cancer and Hyperplasia Biopsy Specimen in Women Aged between 35-65 Years by Indirect Immunofluorescence

Major histocompatibility complex(MHC) class one and intercellular adhesion molecule-I(ICAM-I) play important roles in immune response. It is well known that the expression level of the MHC class I and ICAM-I is frequently altered in accordance with tumor progression and is significantly related to tumorigenesis, tumor progression, and/or metastatic potential(e.g.kidney and prostate cancers). The aim of this study was based on the hypothesis that decreased or absent expression of MHC class I and ICAM-I surface antigens or costimulatory molecules on tumor cells might be responsible, in part, for the progression of breast cancer. Patients & To investigate the expression level of above molecules, 25 breast cancer and 25 breast hyperplasia cells samples from women aged between 35-65 years were obtained. Then, biopsy specimens from samples were prepared. Indirect immunofluorescence technique and H&E staining were used. Immunohistochemical and pathological analysis in this study revealed a negative correlation between the expression of MHC-I and ICAM-I surface antigens and grade (differentiation) of breast cancer was compared with breast benign hyperplasia cell samples. Results were expressed as the mean±SD. Student’s t-test and SPSS program were used to assess the differences, and a significant level of P<0.00 was found. In general, tumor with higher grade(cell differentiation) expressed MHC class I and ICAM-I sufrace antigens on breast cancer cell less frequently and intensely. The absence or decreased level of MHC and ICAM-I surface antigen expression on women breast cancer cells is responsible for tumor progression.