The impact of Ovarian stimulation on mouse endometrium: a morphometrical study

The preparation of endometrium for embryo reception is dependent on the ovarianhormones, which are affected by ovarian hyperstimulation procedure.

The aim of this study was to evaluate the changes in morphometrical indices of endometriumby the daily injection of progesterone after mouse ovarian induction.

Adult virgin female mice were selected and divided into control andexperimental groups. Experimental groups were superovulated using human menopasual gonadotropichormone (HMG), and human chorionic gonadotropic hormone (HCG), then they, were subdivided intotwo groups, which one group was also injected daily by progesterone. All control and hyperstimulatedgroups were rendered pseudopregnant by cervical stimulation. Three and four days after the HCGinjection, the samples of uterine horns were aparted and processed for light microscopic studies.

Our results showed that in the progesterone-injected group, the height of surface and glandularepithelium was decreased on day three (17.6±3.55, 10.02±2.6) and day four (16.9±4.24, 1.6±0.84)respectively, and it had low columnar morphology in comparison with the hyperstimulated and controlgroups. Also the intercellular spaces of stroma in progesterone-injected group were narrower than these inthe other groups.

Ovarian hyperstimulation followed by progesterone injection alter the morphometricalindices of surface and glandular epithelium of endometrium, which could affect on its receptivity.