Enhansment of Biodesulfurization activity via the over expression of the oxidoreductase gene from R.erytheropolis IGTS8using pet 21a vector

Biological desulforezation is a microbial procedure which uses manipulated bacterial strains for increasing of desulforezation from petroleum products. The method is being tested by several big oil companies for making a safe fuel and for preventing air pollution one of the methods for enhancement of desulforezation of microbial oil strains is increasing of copy number of oxidoreductase gene. this gene is able to supply the required FMNH2 for desulforezation genes of Rhodococcus erythropolis IGTS8, which is used as a model micro organism in desulforezation researches, was amplified with PCR and was cloned in to the PTZ57R vector. Then the oxidoreductase gene was extracted by agarose gel DNA extraction kit as a 600 bp fragment and was inserted in to the pkk223-3 expression vector. After induction, the desired protein was weakly detected as a 25 KD band in SDS-PAGE gel. However using another expression vector(PET 21a), a sharp band was detected in the same area. At present, the studies was focused on increasing the production in National Instutute of genetic Engineering and bio technology we are trying to apply genetic Engineering tools to increase gene dosage of oxidoreductase gene to enhancement of desulforezation gene expression