Identification, cloning and structure analysis of β-1,3 glucanase (bgn1) gene from Trichoderma virens (10)

degrade the β-1,3 glucan a biopolymer into its component residues by breaking the β-1,3 glycosidic bonds. As a producer of a variety of glucacase enzymes, the filamentous fungus, Trichoderma, has become an important means of biological control for fungal diseases. The bgnI gene encodes one of the glucanase enzymes which can be used for degradation of β- glucan rich cell wall of various fungal phytopathogenes. In this study two specific primers (RbgnI/FbgnI) and genomic DNA from T. virens (10) with high β-1,3 glucanase activity were used for bgnI amplification. The amplified DNA fragment (about 2.3 kb) was analyzed by restriction pattern using HindIII, pstI, SalI, EcoR1, BamHI and HindII enzymes. BamHI, pstI, HindII and SalI demonstrated unexpected pattern. The amplified fragment was cloned into pUC19 and designated as pUCRM1. The cloned DNA fragment was sequencedand compared with those deposited in NCBI, confirmed the sequence of bglI. This gene is 2345 bp long and contains a short (65 bp) intron and open reading frame encoding a polypeptide with 760 amino acid and molecular weight of 79.4 kDa. The deduced polypeptide shows 60 to 91% homology with other reported related polypeptides.