Serological identification and Purification of Turnip Mosaic Virus (TuMV) in the oil - seed rape

Brassica napus (Canola) with mosaic symptoms from Varamin (Khaveh), was fixed on B. rapa in green house. The samples were collected and tested against TuMV by D.A.Sandwich ELISA using specific antibody (DSMZ, Braunschwieg, Germany). Mechanical inoculation of ELISA positive samples caused necrotic local lesions on Nicotiana tabacum L cv. White Burley and Chenopodium amaranticolor Coste & Reyn, systemic mosaic on - Petunia hybridia Vilm, Nicotiana glutinosa L., Brassica pepo L., Brassica rapa L., Zinnia elegans L.. Purified sample of virus revealed in the sucrose density gradient. Using spectrophotometery at wave lengths of 260 nm and 280 nm the purified virus Varamin of isolate had an absorbance ratio of 1.4. Antiserum was produced by injection of purified virus to New Zealand white rabbit. Electron micrographs of negatively stained row suspension of infected plants and purified virus preparations revealed flexuous, filamentous particles. Iranian isolate virus was transmitted from TuMV – infected plants to healthy plants by Brevicorye brassica in a non – persistant manner.