Comparison of Pseudomonas syringae pv. syringae van Hall Isolates from Different Hosts Based on Phenotypic Characteristics, Serological Properties and Pathogenicity

In order to compare Pseudomonas syringae pv. syringae (Pss) isolates from cereals, citrus, stone fruits and weeds by phenotypic characteristics, serological properties and pathogenicity, samples were taken during 1998-1999 in Fars Province, Karaj, Fereidan, Aligudarz and Shahrekord regions. From 350 fluorescent isolates, 47 were negative with respect to oxidase, potato soft rot and arginine dihydrolase tests but positive in hypersensitive reaction (HR) on tobacco, pelargonium, or both. In complementary biochemical tests, these isolates were identified as Pss. Based on LOPAT (levan, oxidase, potato soft rot, arginine dihydrolase and HR on tobacco leaves) tests, the isolates were divided into two groups, but based on the results of GATTa tests alone, isolates were divided into nine groups. Isolates from different hosts showed differences in lipolysis, syringomycin production, ice nucleation activity, lecithinase, pathogenicity, serology and protein profiles.