The effects of vitamins injection (A, C, A+C and AD3E) on wound healing process and some hematological response in common carp, Cyprinus carpio

This study investigated some hematological response and healing of experimentally wounded carp with injection of vitamins A, C, A+C and AD3E under laboratory conditions at temperature of 17-18ºC, in order to improve the healing process in skin lesions. Ten treatment groups of fish (body weight 120±20g) were injected with 10000, 20000, 50000 IU vitamin A/KgBw, 200, 500, 1000 mg vitamin C/KgBw, 20000 IU vitamin A and 500 mg vitamin C/KgBw, 1ml vitamin AD3E (contains: 5000 IU vitamin A, 10000 IU vitamin D3, 20mg vitamin E)/KgBw, 50mg oxytetracycline/KgBw and one group without any injection as control. We also used 50mg oxytetracycline injection/ kg bw on all treatments. Wounded tissues and blood were sampled in 0, 5, 10, 15, 20, and 30 days after making the incision. During the 30-day period of wound healing, except in differential leukocyte counts, there were no statistically significant changes in hematocrit, total erythrocyte and leukocyte counts. There were also no histological differences among all treatments. Histological analysis revealed all treatment incision site healed 30-day at the same rate. Therefore, injection of vitamins did not improve wound healing in common carp under the conditions of this study.