Effect of Fibroblastic Growth Factor on Resumption of Meiosis In Vitro Maturation and Embryo Development of Immature Mouse Oocytes

The purpose of this study was to evaluate the effect of fibroblastic growth factor on resumption of meiosis in vitro maturation of immature mouse oocytes and resulting embryo development with and without basic fibroblastic growth factor-4 (bFGF-4). cumulus – oocyte complex (COCs) and germinal vesicle (GV) were obtained from female NMRI mice 46-48 hours after administration of an intra-peritoneal injection of 5 IU PMSG. COCs were cultured in TCM199 supplemented with different dosages of bFGF-4. After 24 hours metaphase ІІ (MІІ) oocytes were co-incubated with sperms for 4-6 hours in T6 medium. For all groups the rate of cleaved embryos was assessed in the T6 medium until blastocyst stage In all compared groups the percentage of matured MII oocytes in the10 ng/ml (%94.4) and 20 ng/ml(%92.5) of bFGF-4 treatment groups was significantly higher