An in vitro comparison of microleakage of two self- etch adhesive and the one- bottle adhesive used in pit and fissure sealant with or without saliva contamination

Complete control of intra- oral moisture is difficult to achieve. To minimize the deleterious effects of contaminated enamel on bonding, some authors have advocated the use of adhesive systems under the sealant. The aim of this study was to compare the microleakage of two self- etch adhesive and the one- bottle adhesive used in pit and fissure sealant with or without saliva contamination. Methods and Subjects: Sixty extracted non- carious upper premolar teeth were assigned to the six groups of 10 teeth. Group1: acid + saliva+ Single Bond+ sealant, Group 2: acid+ Single Bond+ sealant, Group3: saliva + S3 Bond + sealant, Group4: S3Bond + sealant, Group5: saliva+ Protect Bond+ sealant, Group 6: Protect Bond + sealant. The teeth were thermocycled (1000 cycles, 5-55º c) and immersed in 2% basic Fuchsine dye for 24 hours. Then the teeth were sectioned and examined with a stereomicroscope under X40 magnification. The results were evaluated with Kruskal – Wallis and Dunn. Group 2 had lower microleakage scores than group 4 and 6. Also, group 6 has the lower microleakage scores than group 4 and generally an increase in microleakage was observed in each group related to contamination. The findings showed that the best technique of sealant therapy in saliva contaminated and non- contaminated condition is the use of acid–etching and bonding agent and so the use of self-etch adhesive, is not advised.