The effect of ketoconazol chronic administration on histomorphometric changes of testicular tissue in mice

Ketoconazol is an important antifungal drug. This drug, in addition to being antifungal effect, has an inhibitory effect on steroid hormones such as sexual hormones & glucocorticoids. According to recent studies the usage of ketoconazol causes to reduce the amount of blood testosterone concentration and different changes on testis tissue in laboratory animals. The study aims at investigating ketoconazol chronic administration on histomorphometric change of testicular tissue in mice. In this study, 50 male mice were used (5 groups and 10 mice in each group). Animals were received ketoconazol (50 mg/kg body weight) daily orally administration, during 15 days, 1, 2, 3 months. The control group was taken normal saline and four groups were treated with ketokonazol. The testis tissue samples were collected and tissue processing were done and stained with H&E. Then, the histomorphometric changes of testicular tissue which include, seminiferous tubules diameter, seminiferous epitelial thickness, interstitial tissue and testicular capsule thickness were studied. Results showed that seminiferous tubules diameter, seminiferous epitelial thichness and interstitial tissue thickness had not significantly changed in the 15 day, but the first, second & third months after drug administration, they had significantly changed (p<0.05) compared with control group. Our results showed that seminiferous tubules diameter, seminiferous epitelial thickness and interstitial tissue thickness in mice testis were changed after ketokonazol administration. This results probably obtained by reducing of serum testosterone concentration.