QTL Analysis of Forage Quantity and Quality-Related Traits of Barley

In order to map the genomic regions affecting barley forage quantity and quality, two experiments were conducted with 72 doubled haploid lines and their two parents (‘Steptoe’ and ‘Morex’), at the Research Farms of the Faculty of Crop and Animal Sciences, University College of Agriculture and Natural Resources, University of Tehran and Agriculture and Natural Resources Research Station of Sistan, in 2007. The experiments were arranged in a randomized complete block design with two replications. Each plot consisted of six rows that were 3m in length and spaced 25cm apart. QTL analysis was conducted by Composite interval mapping (CIM) method separately for each trait in each location. The main effect of genotype was high significant for all the studied traits. Transgressive segregation in both directions (positive and negative) was observed for all the studied traits. There was a negative relationship between forage qualityrelated with quantity-related traits. Thirty-three QTLs controlling different studied traits were identified. Phenotypic variance explained by these QTLs varies from 7.07 to 39.04%. Highest LOD scores were obtained for the leaf to stem ratio on chromosome 2H. QTLs of forage quality (total digestible nutrient, dry organic matter digestibility, leaf to stem ratio, seed to forage ratio and number of tiller per plant) and quantity (plant height, forage wet and dry matter) indexes were found on chromosomes 1H, 2H, 3H, 4H, 5H, 6H and 7H. Most of mapped QTLs appear to be fairly stable between locations and can become candidates for marker-assisted selection.