The Effect of Silybum marianum on GFAP and Spatial Memory in a Mouse Model of Alzheimer

Introduction & Studies have shown that Silybum marianum have high levels of antioxidant polyphenolic substances and have neuro-protective effects on neurodegenerative diseases. Accordingly, this study was conducted to determine the possible effect of Silybum marianum on expression of and spatial memory in a mouse model of Alzheimer's disease. This experimental study was conducted at Yasuj University of Medical Sciences in 2009. Thirty adult male Wistar rats were allocated in three groups: sham group, experimental group, and lesion group, each consisting of ten rats. The experimental and lesion groups received Ibotonic acid of the NBM nucleus in stereotaxic apparatus whereas the sham group underwent surgical procedure without any injection. The experimental group received 200mg/kg of Silybum mirianum extract orally, diluted in 1% Arabic gum. Also the sham group received 1% Arabic gum every day for four weeks. The lesion group did not receive anything. The behavioral assessment was measured, after treatment, by using of Y maze test on day 7 and 28 in all groups. The ELISA method was used to measure the GFAP level in Hippocamp at the end of behavioral assessment. The collected data was analyzed by the SPSS software using ANOVA and Repeated Measures of Analysis Variance tests.Improvement of behavioral performance of the experimental animals compared to the lesion and sham groups were increased significantly on day 7 and 28 (P <0.01 & P <0.001 respectively). The ELISA method showed that the level of the GFAP synthesis decreased in the experimental group compared to the lesion and sham groups (P <0.001). The Silybum marianum plant has a protective effect on the nerve tissue in a mouse model of Alzheimer's disease by decreasing of the GFAP synthesis and lead to the improvement of behavioral performance.