Rapid Diagnosis of Marek's Disease in Chicken by Using a PCR Method.

The main aim of this study was to evaluate a new PCR method for diagnosis of Marek's disease that uses a part of MDV viral genome named Thyamidine kinase (from base 597 to 807) and it could be found in MDV RB1B and its serologic dependent vaccine HVT Fc-126. High performance and time providence in this method makes it a good diagnostic tool for using in laboratories. This is a rapid method that users need only half an hour for sample preparation, 3 hours for PCR and 1 hour for electrophoresis. During this survey 12687 slaughtered broilers in poultry slaughterhouse were studied. Eight macroscopic & microscopically cases found positive showing MD changes after necropsy. After preparing & staining slides with Hematoxilin & Eosin, sampled broilers had histopathologic changes in skin, liver and spleen. In all 8 cases lymphoid tumors found in skin, in 3 cases in liver and in 1 case lesion were found in spleen. After applying PCR method and electrophoresis, in one of these samples (control) no nucleotide band observed in 200bp area and this case marked negative but it was positive in other 7 histopathologically samples and distinct nucleotide bands were formed in 200bp area.