Hematopoietic effects of [Fe3O (OAc)6 (H2O)3]Cl complex in female Balb/C mice

Iron is an inorganic element that is essential for normal body growth; for example, it is necessary for hemoglobin formation, hematopoiesis and blood oxygen transfusion. After consuming iron, it is absorbed into blood and binds to blood transferrin and is transported to bone marrow for red blood cell production. Some harmful effects of iron consuming include gut excitation, nausea, digestive pains and constipation. So, by using an injectional form of iron some of these unwanted effects might be reduced or eliminated. [Fe3O (OAc)6 (H2O)3]Cl complex is a Fe() complex which was dissolved in physiological serum for interperitoneal injection. We studied its hematopoietic effects in Balb/C mice strain. The complex was synthesized in Department of Chemistry in Teacher Training University and its IR spectrum was similar to the references and its elemental analysis resulted Fe3C12H24O16Cl. In this experiment we used female Balb/C mice in three groups as control, sham and experimental. The mice were kept in suitable conditions and 23C temperature. 300 mg/kg B.W. of solution was injected to each experimental animal once a day for 5 days intraperitonealy (I.P.). The sham groups physiological saline was injected I.P. once a day for 5 days. Mice were sacrified after 3 days and their blood were analyzed for some hematological factors such as hematocrit, hemoglobin, RBC, MCV, MCH, MCHC, WBC, seg. neutrophils, band cells, eosinophils, basophils, lymphocytes and monocytes. The results were analyzed using SPSS software the findings revealed that hematocrit, hemoglobin, MCV, MCH, MCHC and the number of RBC in experimental groups increased significantly without posing any morphological effects. It is concluded that iron in this complex can increase hematopoiesis, after it is absorbed into the blood, binds to apotransferrin to carry in blood and separates in bone marrow, when it is necessary.