Molecular study of frequency of Herpes simplex virus type 2 in Esfahan and Chaharmahal-va-Bakhtiari provinces in year 1388

Infections due to Herpes simlex virus type 2 (HSV-2) often causes genital herpes in men and women, infant herpes and nonsupporative meningitis. These diseases are caused in relation to HIV infection, and might be transfected from mother to baby.In this study the prevalence of HSV-2 was determined for the first time by PCR method in clinical samples collected from Isfahan and Chaharmahal-va-Bakhtiari provinces. One hundred HSV-2 infected sera with high titer of IgG and IgM were collected from suspected patients. In total 82 samples from Isfahan province and 18 samples from Chaharmahal-va-Bakhtiari province were collected. Viral DNA was extracted and PCR was performed by using specific primers for gD gene of HSV-2. The positive results of HSV-PCR and the 1013 bp segment of HSV-2 gD gene was detected in 8 of 100 (8%) of serum samples. In Isfahan province 6.09 % and in Chaharmahal-va-Bakhtiari province 16.66 % were positive for HSV-2. The results of this study demonstrated the prevalence of HSV-2 in studied region similar to other regions of the world. Besides, PCR method is presented as a useful procedure for detection of HSV-2 in infected sera.