Genetic Diversity in Barley Genotypes: I. Seed Storage Proteins (Hordeins) and Agronomic Traits

To study the genetic diversity among barley genotypes, 64 genotypes provided by the Seed and Plant Improvement Institue, Karaj, Iran were assessed for their 22 agronomic characteristics and seed storage proteins. Polypeptide-patterens of storage proteins were determined using SDS-PAGE, and three hordeins (B, C and D-hordeins) were analysed by Photo Capture software. Ca lculated Polypeptide bands based on the molecular weight ranged from 30 to 120 KDa. High genetic polymorphism observed in agronomic traits and polypeptide bands, producted 51 polymorphism bands the greatest belonging to B-Hordein with 22 bands. Clustering analysis using UPGMA method based on Euclidean, agronomic traits were divided to 6 groups, and polypeptides bands based on Jaccard coefficient to 11 groups. The high polymorphism between agronomic traits and polypeptides bands indicated high heterogenousity in these genotypes. Histogram of B-hordein was the most frequent hordein with highest impact on protein-wise polymorphism.