Histological and histomorphometrical study on bone formation around titanium implant with and without Bio-oss in medullary space of rabbit's tibia

Nowadays, various biomaterials have been presented for regeneration of osseous defects and Bio-oss is one of them. The objective of this study was evaluation of Bio-oss function in intramedullary space of rabbit`s tibia near titanium implant. In this study, 12 white New Zealand rabbits were selected and randomly divided into control and experimental groups. These groups based on defined periods were subdivided into 2 triple groups. A defect was made by drilling proximal tibia of each rabbit. The size of defect was equal to implant diameter. In experimental group, at first, defects were filled with Bio-oss and then titanium implants were placed. In control group, only implants were inserted. After 8 and 16 weeks, bone tissue specimens were prepared through undecalcified resin section method and evaluated histologically, histomorphometrically and statistically. After 8 weeks, mean of bone to implant contact (BIC) and the ratio of immature bone surface area to total new bone surface area reached to 5.3% and 94.3% in control group and to 21.4% and 84.7% in experimental group respectively. After 16 weeks, above-mentioned variables reached to 9.6% and 73.6% in control group and to 49.4% and 51.7% in experimental group respectively (p<0.05). Bio-oss could have the bone-healing capacity in medullary space of tibia and could increase the stabilization of titanium implant.