The effect of eye drop route vaccination scheduling with Newcastle Avinew vaccine on stimulating of humoral immunity by Hemagglutination Inhibition test

Newcastle disease is one of the infectious diseases in birds. Doing an accurate vaccination in a proper time is an approach to prevent the disease. This study clarified theeffect of eye drop route vaccination scheduling with Newcastle Avinew vaccine on stimulating of humoral immunity in broilers by Hemagglutination Inhibition testin order to find a better vaccination protocol. So, 150 oneday- old broiler chicks were divided into 3 separated groups. Treatment groups were consisted of A1 (eye dropvaccination at 1st, 14th day old regardless of maternal antibody), A2 (eye drop vaccination at 7th, 17th day old with considering maternal antibody) and controlgroup(without vaccination). Results showed the decreasing trend at 1st,7th,14th days old in all groups and increasing trend after 21th day in groups A1 and A2.Control group had the decreasing trend in all bleeding stages and reached to the lowest level at 35 th days old. Statistical analysis revealed no significant differencebetween A1 and A2 groups (p<0.05). Also, there was no significant difference between control group and the other groups up to 14th day old. However, considering theresults of this study, eye drop vaccination at 1st day of life in high risk regions should be implemented in order to prevent Newcastle disease, regardless of maternalantibody.