Designing of Human Cartilage Tissue, by Differentiation of Adipose- Derived Stem Cells with BMP-6 in Alginate Scaffold

Author(s):

Hashemibeni B. , Razavi Sh. , Esfandiari E. , Karbasi S. , Mardani M. , Nadali F. , Sadeghi F. , Haghighat F. , Hahgju Sh. , Goharian

Abstract:
Purpose
In the present study the effect of BMP-6 was investigated on chondrogenesis of adiposederived stem cells.
Materials And Methods
Mesenchymal stem cells derived from subcutaneous adipose tissue were cultured on alginate scaffold to induce chondrogenesis in experimental group, with chondrogenic medium having BMP-6 growth factor for 3weeks. In control group medium without BMP-6 was applied. The harvested constructs were examined with immunohistochemical and RT-PCR methods for assessment of cartilage–specific characteristics.
Results
The results of immunohistochemical method revealed the presence of typical cartilage extracellular matrix components such as type II collagen and aggrecan in constructs induced by BMP-6 growth factor on alginate scaffold. In addition evaluation of the results of RT-PCR analysis confirmed the expression of cartilage- specific genes, such as type II collagen and aggrecan, in the differentiated cells under the influence of growth factor BMP-6.
Conclusion
It can be concluded that BMP-6 promotes chondrogenesis of ADSC in 3-D and adipose-derived stem cells could be used for cartilage tissue engineering.
Language:
Persian
Published:
Anatomical Sciences Journal, Volume:8 Issue: 2, 2011
Page:
118
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