The effect of Satureja Khozestanica essence on inhibition of glomerular changes in uninephrectomized diabetic rats

Diabetic nephropathy is the common cause of leading to end stage of renal disease.Satureja Khozestanica essence for the first time used as antioxidant, antidiabetic and anti-inflammatory agent for inhibition of diabetic nephropathy. 40 male rats were uninephrectomized from left flank. The rats were divided into four groups randomly(ten per each group), group one as control, group two diabetic without treatment and groups three and four, treatment with Satureja essence with 250 ppm or 500 ppm in drinking water respectively. Diabetes was induced in the second, third and fourth groups by alloxan injection subcutaneously. After 8 weeks treatment of serum malondialdehyde was measured. Kidney paraffin sections were prepared and stained by Periodic Acid Schiff method.Glomerular volume, intraglomerular mesangial volume and volume of glomerular capillary were estimated by stereological rules. Data were compared by SPSS13 software and Mann Whitney nonparametric test at p<0.05. Glomerular hypertrophy, mesangial expansion and reduce of glomerular capillary volume in the groups that treated by Satureja essence, significantly were inhibited in comparisor with diabetic untreated group but it cannot be saved at level of control group significantly(p<0.05). The level of serum malondialdehyde in treated groups was maintained at the level of control group significantly (p<0.05). Satureja Khozestanica essence significantly can inhibit glomerular hypertrophy, mesangial expansion and save glomerullar capillary volume in diabetic rats significantly.