Survey Prevalence and Resistance to Some Beta lactame Antibiotics in Bacillus cereus strains Isolated of AZZAHRA Hospital (Iafahan/1384-86)

Hospital infection is one of important agent morbidity and mortality in patient. According to report of WHO on of average 8.7% of patients in hospital acquired hospital infection. Bacillus cereus is one of agent of hospital infection and cause systemic‚topical and food poisoning. Surface can serve as reservoirs of potential pathogen and is important in chain of infection; staff hands are more source to transmission bacteria from surfaces. Hands of staff are the most sources to transfer bacteria in environment and in patients. The research was performed with laboratory method and performed in 1384/86 years, in Azzahra hospital in Isfahan, according to statistical formula randomly selected 274 (194 sample from surface and 80 sample from staff hand). Environmental samples collected, with swab in NB and staff hand, samples collected whit finger print method, identification bacteria, were performed with microbiological staining, chemical test, use of differential and Bacillus cereus selective media. Survey antibiotic pattern performed with Kirby Bauer method, then data analyzing whit SPSS (ver 14) softwear. Survey result of 247 Bacteria, prevalence of B.cereus strains was %9.49. Prevalence of Bacillus cereus strains in environmental and staff hands were respectively %6.7 and %16.25. According to antibiogram test %81 of strains were resistant to penicillin, %47.6 to cefotaxime and %19 ampicillin. According to high prevalence of B.cereus strains in hospital and their resistance to β-lactame antibiotics, it is necessary for performed grant study in all of hospital in Iran and go on to, controlic methods for reduce prevalence rate, hospital infection and antibiotics resistance.