Frequency of Beta Lactamase Enzyme in Isolated Pathogen Bacteria from Hospital In-Vivo and In-Vitro Condition

Hospital surfaces and Staff hands are most common sources to transmission and spread Bacteria from hospital. β-lactame antibiotics are very important agents to control nosocomial infections. The spread of β-lactamase bacteria can disrupt the treatment process. This study was done to evaluate the frequency of β-lactamase bacteria isolated from staff hands and hospital surfaces. This study was a laboratory study and was performed in the years 2005 to 2007 in Al-Zahra (SA) hospital in Isfahan. 274 samples were selected randomly from stuff hands and hospital surfaces. Hospital surface samples were collected with swab and NB (Nutrient Broth), from high and low contact surface and staff hand samples were collected with Finger Print method. Bacterial identification was performed with microbiological methods and β–lactamase production survey was performed with Acidometric method. Staphylococcus species were seen in 55% of 194 isolated Bacteria from hospital surface. Other bacteria that isolated from hospital surface were Bacillus species in 26.29%, Enterobacteriaceae in 9.80%, Pseudomonas species in 3.9%, other gram negative Bacteria in 4.51%, Streptococcus species in 0.5% of samples. 60% of 80 isolated Bacteria from staff hand were Bacillus species. Staphylococcus species in 36.25% and Enterobacteriaceae in 3.75% were seen in stuff hands samples. According to β–lactamase test result, respectively 75.43% and 71.23% of isolate Bacteria from hospital surfaces and staff hands, produced β-lactamase The results of this study have shown the high frequency of β–lactamase enzyme in isolated Bacteria from staff hands and hospital surfaces. Improving quality of hand washing and disinfectant agent are very important methods to control nosocomial infections.