Effect of Amniotic Fluid in Treatment of Post-Laparatomy Adhesion Formation in Rat Model

Intraperitoneal adhesion is one of the most important complications after laparatomy and it may cause many problems for patients in long time such as infertility, bowel obstruction, and chronic pelvic pain. This study was done to determine a way to reduce the formation of adhesion after laparatomy. In this experimental and laboratory study, 40 adult female rats divided in two equal groups, randomly (case and control). In stage 1, all of the rats were anesthetized. Then, we opened the abdominal wall; 6 about 2-cm incisions were made on the right side and in left, a 1 × 1-cm piece was excised from abdominal wall. In stage 2, after 2 weeks we opened abdominal wall and according to Cook scale, we scored adhesion banding; then we added 3 cc amniotic fluid in case and 3 cc distilled water in control group. In stage 3, after 2 weeks, we opened abdominal wall by midline incision and adhesion were scored. Data were analyzed by tests such as t-test and paired t-test. The adhesion was less in case group (P < 0.001). Also, the difference between grades of two groups was significant (P = 0.02). Adhesion bands after laparatomy is one of the most important problems in surgery. Many material was surveyed to prevent or treat adhesion band such as Normal saline, dextran 70, HAL-F, ePTFE, pentoxyfyline, promethazin…. In our study, we used bovine amniotic fluid to determine. Our study showed that amniotic fluid is capable to lyses of adhesion band after laparatomy. More researches are needed to find out positive effects of this material to use as anti adhesive agent in human.