Evaluation of MYCN amplification in Iranian patients with neuroblastoma by conventional and real time PCR

Author(s):

Behzad Poopak , Mohammad Taghi Arzanian , Parvaneh Vosough , Atoosa Gharib , Amir Ali Hamidieh , Mohammad Ehsani , Masood Parsania , Abolfazl Yousefian , Mohammad Ali Jahangirpour , Kobra Farahani

Message:
Abstract:
Background
There is an international consensus that evaluation of MYCNamplification should be done in all cases of newly diagnosed neuroblastoma. MYCN is the most important prognostic factor for neuroblastoma and determines treatment strategy.
Materials And Methods
In this study we evaluated paraffin embedded tissue block and bone marrow aspiration of 75 neuroblastoma patients with mean age of 4.1 years, including 32 female and 43 male, by conventional and also real time quantitative PCR.
Results
Forty eight and 43 percent were MYCN amplification positive by Real time and conventional PCR, respectively. 28% of patients less than one year old and 48% of patients older than one year showed MYCN amplification.50 percent of cases with pathological diagnosis of small round cell tumor had MYCN amplification.
Conclusion
PCR is a fast, reliable and cost effective method for the evaluation of MYCN amplification and can be performed using DNA extracted from small tissue samples and paraffin embedded blocks. As expected regarding detection power and convenience, Real time PCR was superior to conventional PCR.
Language:
Persian
Published:
Medical Science Journal of Islamic Azad Univesity Tehran Medical Branch, Volume:21 Issue: 3, 2011
Page:
188
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