Changes in the lung HSP72 and superoxide Dismutase after regular physical activity and antioxidant nutrition- Study the effect of pollution

We investigated the effects of aerobic training and antioxidant supplement on heat shock protein (HSP72) and superoxide dismutase (SOD) in lung tissue in rats exposed to lead acetate. Forty, young rats were randomly assigned to control groups (Sham-operate) and treatment groups (Lead acetate, Antioxidant supplement, Aerobic training and Aerobic training + Antioxidant supplement group) (n=8 per group). The treatment groups received lead acetate (20 mg/kg) only, while the antioxidant supplement and aerobic training + antioxidant supplement groups also, received (30 mg/kg) Curcumin supplement peritoneally 3 days in week for 8 weeks. Furthermore, rats in the aerobic training groups performed the progressive running exercise of 15 to 22 m/min for 25 to 64 min, 5 times a week. HSP72 was measured by ELISA in the homogenized lung tissue and SOD, MDA and TAC using standard methods. Data was analyzed by one-way ANOVA test and Tukey at P<0.05 level. HSP72 and MDA were increased significantly in the lead acetate group compared with other groups. On the other hand, SOD and TAC concentrations were significantly increased in the aerobic training, antioxidant supplement and aerobic training+ antioxidant supplement groups compared with lead group. These results suggest that life health method may be important determinants in prevention of oxidative/antioxidant process related to chronic expose to lead acetate.