Determination of unsaturated fatty acids (EPA, DHA) and omega 6 in three species of aquaculture fish, rainbow trout, common carp and silver carp

In order to compare the amount of omega 3 and omega 6 fatty acids in Salmon, Carp and Silver carp, the samples were prepared from market during Jan. and Feb. (2006).With considering the statistic approach for sampling, after extraction of fish oil, they were purified and hydrolyzed. After hydrolysis of oil and isolation of fatty acids, they were esterified and preconcentrated by the evaporation of solvent and injection to GC-MS. As standard, the capsule of omega 3 were prepared and the same process was carried out before injection to GC-MS. The results show that the amount of omega 3 is higher in Salmon whereas Carb has the lowest amount.