مجله دانشکده علوم پزشکی نیشابور
پیاپی 19 (تابستان 1397)

Introduction and Aims: Absorption and distribution of nitrate in agriculture products have significantly important because high concentrations of nitrate in foodstuffs, especially in leaf vegetables, leads to increased risk of stomach and intestinal cancers in adults and methemoglobinemia in infants. This study is done for the first time in a laboratory environment to study the effect of various concentrations of municipal wastewater on nitrate accumulation in leaf vegetables.

This experiment is accomplished as in greenhouse environment. For this purpose, seeds of leaf vegetables including garden cress (Lepidium satrivum) belongs to Brassicaceae and spinach (Spinacia oleracea) belongs to Chenopodiaceae were purchased and sown in greenhouse and were irrigated as 4 treatments: T1 (irrigation only by means of well, “control treatment”), T2 (irrigation with raw wastewater once a week), T3 (irrigation with raw wastewater twice a week) and T4 (irrigation only with raw wastewater). The experiment performed in three replications in order to gain a proper statistical condition. After growth and preparation of samples, the amount of nitrate was measured in edible parts of each of samples by means of JENWAY model of spectrophotometer device at 400 nm wavelength. To compare the average of nitrate concentration in samples, ANOVA test at a level of 5 percent and SPSS software were used.

According to the results, the effect of various wastewater treatments on the amount of accumulated nitrate in leaf vegetables was meaningful. In a comparison of treatments with the control treatment, the most nitrate accumulation was observed in T4 (irrigation only with raw wastewater). The amount of accumulated nitrate in different treatments of vegetables was in the allowable range of world health organization report and lower than the range of toxicity threshold (p < 0/05). The nitrate concentration in different spinach treatments was also further than similar treatment of garden cress (p < 0/05).

In the present study, the amount of nitrate concentration differed in various families of leaf vegetables. Irrigation with municipal raw wastewater increased the nitrate concentration of atmospheric organs of studied leaf vegetables in terms of quality. Due to their harmful effects on human societies and endangering food safety, more precautions are required to reduce such effects

Studies have pointed to the possible role of FOXO1 in the pathogenesis of type 2 diabetes, due to the claim that it affects glycemic control and lipid metabolism. The aim of this study was to investigate the effect of 12 weeks of high-intensity interval (HIIT) on FOXO1 expression in subcutaneous adipose tissue, insulin resistance, insulin resistance, and blood glucose in type 2 diabetic rats. This is a fundamental and experimental research. The statistical population of this study is all male Wistar rats of Pasteur Institute of Iran. Among them, 14 male Wistar 10 week old rats weighing 220 ± 20 gr Randomized method was used to participate in the study. After a fasting night (12 hours), rats were diabetic by injection of nicotinamide and streptozotocin and divided into 2 groups of HIIT training and control. 12-week exercises were performed in 5 sessions of 30 minutes per week in the running of the treadmill with 1-minute repetitions and 2-minute active rest. The untrained diabetic group did not attend any exercise program. 48 hours after the last training session, the rats were anesthetized and slaughtered. A t-test was used for statistical analysis. 12 weeks of periodic exercise decreased the relative expression of FOXO1 in comparison with the control group (P = 0.033). However, there was no significant difference in insulin resistance between the two groups (P = 0.921), but glucose and insulin decreased significantly with 12 weeks of periodic exercise (P = 0.001). 12 weeks of HIIT training leads to a decrease in blood glucose levels, despite the lack of insulin resistance in type 2 diabetic rats. This improvement can be attributed somewhat to signaling pathways dependent on changes in the expression of FOXO1 independent of insulin resistance

Introduction and Although bone regeneration is a complex and systemic physiological process which happened during regeneration remedy, the treatment of bone defect has been a problem faced by the clinical situation. At present, cell therapy is commonly used to treat bones defects. The patterns of cell therapy using stem cells and osteogenic inducing mediators. In the present study, the ability of homeopathy medicine symphytum to induce cell differentiation of rat bone marrow-drivedmesenchymal stem cells (rat’s BM-MSCs) into osteoblasts was studied.
Material and Rat’s bone marrow mesenchymal stem (rBM-MSCs) were isolated from rat’s bone marrow. Cytotoxic effects of homeopathic symphytum on BM-MSCs was evaluated by MTT assay. Intracellular calcification was studied with alizarin red staining after 7 and 21 days of osteogenic treatments. MTT assay results attended homeopathic symphytum could decrease cells growth and proliferation independence of time and potency, 200C has high cytotoxicity (28% viable cells) so that lower potencies (30 C, 12 C, 6C) were used for next experiments. Alizarin red staining showed all the test groups could induce significant variation with negative control. The lowest potency (6 C) was significantly increased the differentiation of mesenchymal stem cells to osteoblast cells and synergism groups in compare with test group have lower color intensity. According to this finding, Homeopathic symphytum could enhance osteogenic differentiation of rBM-MSCs and could be considered as a safe alternative for standard osteogenic medium

Colon adenocarcinoma and hepatocellular carcinoma are among the most prevalent cancers in Iran. Since chemotherapy resistance is a major obstacle in the treatment of these malignancies, it is necessary to introduce novel and more effective anticancer regimens. The purpose of this study was to compare cytotoxicity of parthenolide, a sesquiterpene lactone, with alkylating agents melphalan and procarbazine. To compare toxic effects of parthenolide, melphalan and procarbazine, human colon adenocarcinoma and hepatocellular carcinoma cell lines were used. The viability of HT29 and HepG2 cells was evaluated by MTT assay 24, 48 and 72 h after treatment, and cells were checked for morphological alterations by the end of each time point. The IC50 values of parthenolide and melphalan in HT29 cells were determined as 8.5 and 160 mg/mL, respectively, while the highest concentration of procarbazine reduced cell viability by only 5%. In addition, the IC50 values of parthenolide and melphalan in HepG2 cells calculated as 15 and 80 mg/mL, respectively, while 160 mg/mL procarbazine reduced cell viability by 11%. To note, morphological changes confirmed MTT results in both cell lines. Findings of the current study indicated that parthenolide induced more cytotoxic effects in comparison with alkylating drugs. Since this terpenoid derivative effectively reduced the viability of HT29 and HepG2 cells, it could be considered as a potent agent to design new chemotherapy regimens against colon and liver cancers

Breast cancer is the most common cancer among women and the average age of the disease in Iran is lower than the global average. Nowadays, the use of nanoparticles has become one of the effective therapies for cancer treatment. The purpose of the present study is to use water containing nano-bubble oxygen with a concentration of 98% oxygen as a complementary therapeutic agent for the treatment of breast cancer in affected mice for the first time in Iran BALB/c mice with invasive TNBC-4T1-induced cancer grafts were infected and then mice were fed in two groups of nano-oxygen and nano-air bubbles water for 14 days and Control group (normal water) were compared. Finally, p53 gene expression was compared in three groups using real-time PCR. Reduction in tumor size in the second week as well as ultimate tumor weight loss in mice fed with oxygen nanobubbles were observed in comparison with a control group and increased expression of p53 gene in the nano-oxygen group, possibly due to high concentrations of oxygen in the tissue Cancer was also obtained from the control group. Oxygen nano bubble seems to play a positive role in increasing the expression of p53 gene in comparison with nano-water and common water in inhibiting tumor growth

Atherosclerosis is a major risk factor for the development of cardiovascular diseases. The aim of this study was to determine the effects of kambucha tea on oxidative stress induced by high cholesterol diet in New Zealand rabbits. In this experimental study, 32 New Zealand rabbits (weighing 2500-2000 g) were randomly divided into 4 groups of 8. The first group (control) received a standard diet and the second group (hypercholesterolemia) received a high cholesterol diet (1%) for 40 days. The third group received kambucha tea (gavage-day), and the fourth group received a high-cholesterol diet plus kambucha tea (10ml/kg). Eventually, serum levels of cholesterol, malondialdehyde, antioxidant capacity, and nitric oxide were determined. Group receiving high cholesterol diet plus kambucha tea and group receiving only kambucha tea had significantly lower cholesterol level than the group receiving only high cholesterol diet (p < 0.0001, p = 0.03). Serum levels of MDA (malondialdehyde) in rabbits that received kambucha alone and kambucha plus high cholesterol diet were significantly lower compare with hypercholesterolemic groups (p = 0.001, p = 0.02). The group received the only kambucha, serum antioxidant capacity significantly increase compared to hypercholesterolemic group (p < 0.0001). Serum NO (nitric oxide) levels in rabbits that only received kambucha significantly decreased (p = 0.001). It can be argued that kambucha tea may be able to prevent the progression of atherosclerosis by improving the antioxidant defense system and preventing the oxidation of lipids

Introduction and this study carried out to design and prepare novel double layer electromagnetic Nano composite to control the intensity of X-band frequency range.
Material and this study used the Resin Epoxy and the Nickel Oxide nanoparticles as matrix and filler. Double layer Nano composite plates prepared by single layer Nano composites that were on each other in three different thicknesses and nine various loading weight of filler. Shielding effectiveness were measured using Vector Network Analyzers in X-band frequency range. the highest average of shielding effectiveness was obtained 52.51% in double layers. Shielding effectiveness increased by Changing thickness from 8 to 10 mm and 8 to 12 mm. Enhancement of filler loading and change in the layer arrangement could not affect the shielding effectiveness. In this study, a novel double layer electromagnetic Nano composites were design and prepared using the nickel oxide nanoparticles and Resin Epoxy in X-band frequency range that was able to act like a Half Value Layer. Also, all double layers Nano composites could exhibit attenuation values more than a Half Value Layer. In this research, change in arrangement of layers and increase in total thickness and filler contents had not important role in enhancing the shielding effectiveness. It is recommended that in future studies the effect of type of fillers and integration in structure of double layer shields be investigated

Heart disease has a direct relationship with the increase of LDL-c and decrease of HDL-c, and 8ABCG has a positive effect on these two variables by preventing atherosclerosis by interfering with the reverse transmission of cholesterol. Therefore, this study aimed to compare the effect of eight weeks aerobic training with green coffee consumption on ABCG8, leptin, and HDL gene expression in overweight women.

30 overweight young women were randomly divided into 10 groups and eight weeks of aerobic training with a frequency of three sessions per week, the intensity of 50% of the maximum heart rate, and in the last two weeks severely increased 75% of the maximum heart rate. one hour before From a practice in a one-way blind, group “Practice and green coffee” received one 400 mg capsule and group “Placebo and green coffee” received Placebo. In order to compare the variations between groups was used one-way ANOVA (P <0.05).

The results showed that the values ​​of all three variables ABCG8, leptin, and HDL-C had significant changes (p> 0.05). The LSD post hoc test in all three variables indicates a significant change between “Practice and green coffee” with Other groups.

Exercise and green coffee can affect the health of people by expression of ABCG8, an increase of HDL-C, a decrease of leptin and weight. Therefore obese people can use this type of exercise and complementary green coffee to prevent cardiovascular disease and weight loss