فهرست مطالب

Men's Health Journal - Volume:3 Issue:1, 2019
  • Volume:3 Issue:1, 2019
  • تاریخ انتشار: 1398/10/05
  • تعداد عناوین: 5
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  • Farzad Allameh, Amirreza Abedi, Maryam Karimi, Morteza Fallah Karkan* Page 1
  • Fereshte Aliakbari, Mohammad hossein Heidari, Mohammad Ali Hossini, Jalil Hosseini* Page 2
    Introduction

    Mouse spermatogonial stem cells (SSCs) can be cryopreserved for long periods while preserving their spermatogenic ability. Although cryopreservation has been found to increase re- active oxygen species (ROS) formation that damages cellular structures. In the present study, we added vitamin E to the basic freezing medium in order to evaluate its effect on the efficiency of spermatogonial stem cells.

    Methods

    SSCs isolated from testes of 6 days old male mice by enzymatic digestion. Vitamin E 100, 200, 400 μg/mL was added to the basic freezing medium. The cell viability was evaluated by MTT assay. After thawing, SSCs were cultured for 1 month and the expression pattern of specific genes of SSCs measured by real-time PCR technique.

    Results

    The survival rate of the freeze cells in the presence of vitamin E was significantly higher than the control group (p<0.05). The number of colonies and their diameter measured after one month were significantly higher in the vitamin E groups than in the control group (p≤0.05).

    Conclusion

    Adding vitamin E to the basic freezing medium thus can be helpful in increasing the quality and viability of SSCs after cryopreservation.

    Keywords: Spermatogonial stem cell, Cryopreservation, Culture, vitamin E
  • Mohammadreza Mohammadi Fallah, Ali Tayyebi Azar* Page 3
    Background

    Varicocele means dilation and torsion of spermatic veins. About 15%-40% of infer- tile men suffer from Varicocele. It seems that 70% of secondary infertility in men occurs due to Varicocele. Varicocele is the most common surgical cause for male infertility. Varicocelectomy is considered as the best treatment method for patients with Varicocele that increases 30%-50% of the postoperative fertility rate. The purpose of this study was to investigate the effect of Vari- cocelectomy on inhibin B and use this marker to predict infertility in men.

    Methods

    In this study, all infertile patients in any age that were candidate for Varicocelectomy and hospitalized in Imam Hospital in Urmia were included. Exclusion criteria were defined as or- chitis, trauma, diabetes, radiotherapy, chemotherapy and coitus complications. Sperm analysis (SA) was conducted three times for patients and then serum levels of FSH, LH, T, inhibin B, SA were measured before surgery and also six months after surgery. Furthermore, during the hospi- talization and infertility period, testes were examined before surgery in terms of consistency and size.

    Results

    The mean ± SEM (standard error median) age of 40 patients was 28.4±5.14 (range: 18- 40) years old. Mean size of the testes and mean sperm density and serum levels of LH.FSH re- mained the same. However, mean sperm motility, serum levels of T and inhibin B were statistically significant after the Varicocelectomy (p-value=0.005). A significant relationship was found be- tween serum levels of inhibin B and infertility; however, there was no significant relationship be- tween sperm count and serum levels of LH, FSH and T. Varicocelectomy can improve sperm mo- tility and increase Inhibin B in patients and so improves spermatogenesis parameters and it may be one of the causes of fertility in patients.

    Conclusion

    The results of this study indicate that sperm motility and increase in inhibin B after Varicocelectomy can improve spermatogenesis parameters. Serum levels of FSH, T, LH and sperm morphology and also grade of Varicocele, patient age, and testis size have no effect on spermato- genesis and fertility after Varicocelectomy.

    Keywords: Inhibin B, Varicocele, Follicle Stimulating Hormone, Luteinizing hormone, Testosterone
  • Mansoureh Vahdat, Samaneh Rokhgireh, Elahe Afshari, SamanehMohammadpour, Mohammad Ali Hosseini* Page 4
    Introduction

    Uterine septum is one of the most common congenital abnormalities in women that leads to numerous gynecological problems and adverse obstetrics outcomes. This study aimed to evaluate the effects of Hysteroscopic Resection on pregnancy outcomes in women undergone the surgery.

    Methods

    In this quasi-experimental study, 90 women were included from April 2016 to June 2018 from patients attending to Rasoul Akram hospital of Tehran. The inclusion criteria included: the age lower than 35 years old, primary infertility, idiopathic recurrent spontaneous abortion, BMI between 19 and 30, and having informed consen. Septum was resected by scissor upward and lateral. After 10 months of follow-up in average, we assessed rate of live births, abortions, birth weight and presentation.

    Results

    82 individuals were assessed for occurrence of conception (response rate=91%). The mean age of patients was 30.01 ± 6.76 years and the mean BMI was 26.25 ± 4.88. Out of 82 patients, 36 patients were pregnant, of whom 16 (44.4%) had abortions. 5 (14.9%) of the pregnancies ended with preterm birth, and 6 (17%) ended with stillbirth.

    Conclusion

    The present study showed that the infertile patients with uterine septum and with no other causes of infertility were more likely to be pregnant compared to other patients with idiopathic infertility. Our study showed that post-operation fertility following Hysteroscopic Resection was lower than that in previous reports. According to the findings of this study, scissors may be safe, effective and cost-effective method for removing uterine septa.

    Keywords: Septoplasty, Septate uterine, Recurrent abortion, Infertility
  • Maryam Khayamzadeh+, Fereshte Aliakbari+, Zahra Zolghadr, MajidEmadeddin, Afshin Moradi, Mohammad Esmaeil Akbari, Amir Reza Abedi*, Shahrzad Nematollahi*, Jalil Hosseini Page 5
    Introduction

    Bladder cancer is the ninth common cause of cancers in both sexes worldwide. Nevertheless, little is known about the descriptive and analytic epidemiology of bladder cancer in Iran. The present study aimed to describe the nationwide distribution of death due to bladder cancer in Iran.

    Methods

    This cross-sectional study used data of bladder cancer cases who were registered in the national cancer-registry system by the Ministry of Health and Medical Education during 2001-2007. Age-standardized mortality rates due to bladder cancer were presented according to nine geographic poles across the country.

    Results

    The overall mortality rate of bladder cancer (per 100,000 population) was 2.26 in men and 1.36 in women; while the rates were constantly higher for men across all age groups. The highest and lowest age-standardized mortality rates in provinces (per 100,000 population) belonged to Mazandaran (6.126) and Tehran (1.112), respectively.

    Conclusion

    Death from bladder cancer seems to increase by age in Iran, mainly among men. This association might be partially due to increased life expectancy, altered high-risk lifestyle behaviors and/or improvement in cancer registration system. Information on the distribution of mortality due to bladder cancer could be useful for local prevention strategies, where specific profile of communities and patients is taken into account

    Keywords: Urinary Bladder Neoplasms, Mortality, Registry