مجله سلول و بافت
سال دوازدهم شماره 4 (زمستان 1400)

The aim is to investigate the effects of magnetic nanoparticles on tissue health.

Magnetic iron nanoparticles were produced using Fusarium oxysporum and after proving their size to evaluate their effect on the liver tissue of 24 rats, they were randomly selected and divided into 4 groups. Based on the interventions, nanoparticles were injected intraperitoneally. At the end of the experiment, tissue sampling was performed and the samples were sent to the laboratory to prepare histopathological sections and analyze induced paired plasma spectroscopy.

The fungus produced magnetic iron nanoparticles and visible light spectrophotometer tests, X-ray diffraction confirmed its presence and electron microscopy showed that their average size is 20 to 30 nm. MTT test also showed that magnetic iron nanoparticles have low toxicity and ICP analysis showed that with the presence of electromagnetic field, the rate of entry of iron nanoparticles into the tissue has increased. Microscopic results also showed that the most changes in hepatocytes, lobular center vein and sinusoidal space were in the electromagnetic field group and non-toxic dose group of nanoparticles with the presence of electromagnetic field.

Based on the results, it can be said that while magnetic iron nanoparticles do not induce a specific toxic effect in tissue, but the probability that the electromagnetic field itself causes tissue changes increases.

Aim Regarding to prevalence of corona disease worldwide, evaluating of therapeutic strategies for this epidemic is very valuable. The aim of this study, was to introduce different strains of coronavirus, to investigate the cause of SARS-CoV-2 disease and cell therapy strategies using stem cells and extracellular vesicles derived from stem cells. This research is considered as a basic and descriptive- analytical on the basis of research purpose and method. In this study, the keywords including corona, cell therapy, mesenchymal stem cells and exosome were used. The search was conducted in the Google Scholar, PubMed, SCOPUS, ISI Web of Knowledge databases. The investigation period was also considered from December 2019 to June 2021. So far, seven species of the coronavirus family have been identified that can transmit to humans. In a recent report, researchers looked at stem cell- derived systems to further investigate the emerging form of beta-coronavirus, SARS-CoV-2 in the body. Cell therapy using stem cells is a valuable option; because these cells have functional immunity by creating organoids in the culture medium and maintaining signaling polarity. Therefore, in the two- or three-dimensional culture model, the use of stem cells has become an applied model for studying the pathogenicity of the virus. Evantually, exosome derived stem cells are another candidate with regenerative potential on injured lung which was considered as valuable therapeutic strategy in these disease.

Aim Considering that significant progress has been made in the field of cryopreservation and long- term storage of sperm in different species, but this process causes significant damage to the sperm cell. In this article, we have tried to review the studies related to sperm storage tanks in poultry and their role in sperm function. The researchers concluded that with a proper understanding of how sperm is stored in the oviduct, sperm can be stored for longer without freezing. This is in favor of species whose sperm have a shorter viability after freezing and can only be stored in liquid conditions for a few days. Compounds called glycans in the epithelium of the oviduct and sperm binding proteins to these glycans are more commonly referred to. In this review, information about sperm storage tanks, the role of these tanks in sperm function, glycans were studied in mammals from google scholar, pub med, andrology, reproduction of domestic animals and biology of reproduction. By studies of these contents, we conclude that in some species, sperm are stored for a long time in the female reproductive system and are able to produce offspring without the presence of males, by carefully studying the mechanisms involved and being inspired by these events, we can succeed in preserving superior genetic resources and preserving the sperm of endangered species without freezing.

The main objective of this study was to develop and characterize NRG-loaded solid lipid nanoparticles (NRG-SLNs) on the breast cancer cell line (4T1).

NRG-loaded solid lipid nanoparticles were prepared by hot-melt emulsification and high-speed homogenization method. The cytotoxicity of NRG-SLNs was evaluated by measuring the viability of 4T1 cells using the MTT assay. Additionally, the induction of pro- apoptotic proteins and suppression of anti-apoptotic ones by NRG-SLNs were analyzed through using Western-blotting method.

The prepared NRG-SLNs had mean size of 84 nm, zeta potential of −21 mV and the entrapment efficiency of 73.17%. IC50 values of 4T1 cells treated with NRG-SLNs were 20 μM at 24 h and 2 μM at 48 h of incubation. The western blot analysis showed that NRG-SLNs could increase Bax / BCL2 ratio compared to the control group. Based on the results, level of Bax, p-ASK1 and cytochrome c enhanced in the treatment group.

In conclusion, NRG-SLNs may have the potential to inhibit growth and induce apoptosis in the breast cancer cells of 4T1.

In this study, investigated the effect of flavonoid compounds in walnut skin on the production of insulin-secreting cells.

In this experimental study, adipose-derived mesenchymal stem cells (AD- MSCs) were differentiated into insulin-producing cells under sterile conditions for 21 days in the presence of flavonoid extract at doses of 50 and 100 mg/ml. Streptozotocin at a dose of 60 mg/kg was used to diagnose rats. Dithizone staining (DTZ) for insulin, Immunofluorescence to determine the presence of pancreatic beta-specific proteins, Blood glucose measurement by glucometer, Serum creatine, urea and uric acid levels by calorimetry and Reverse chain reaction transcription E- polymerase (RT-PCR) was used to evaluate the expression of PAX4 gene.

AD-MSCs under the above conditions gradually changed from spindle-shaped fibroblasts to circular cells and showed insulin-producing cells using red DTZ dye and insulin secretion .Expression of insulin-pronsulin and beta receptor markers was demonstrated, blood sugar, urea, uric acid and creatinine levels decreased and the expression level of PAX4 gene in experimental groups showed a significant disorder (P≥0.05).

The results showed that AD-MSCs under the flavonoid extract of walnut green skin have the ability to differentiate into insulin-producing cells. Keywords: Mesenchymal stem cells, Insulin-producing cells

The aim of this study was to compare the effects of chloroform extract of two types of red and brown algae on proliferation, cell cycle and induction of apoptosis in breast cancer cell line.

In this experimental study, MCF7 cancer cell line and normal MRC5 cell line were cultured and then the cells were treated with different concentrations of chloroform extract of algae for 48 hours. Cell survival percentage was calculated by MTT method and type of cell death and cell cycle arrest was assessed by flow cytometry. The results were evaluated by Prism software.

The results showed that both brown and red algae reduced the proliferation of MCF7 cancer line as a concentration-dependent pattern. The IC50 concentration was 88 μg/ml for brown algae chloroform extract and 107 μg/ml for red algae. These values indicated more inhibitory effects of brown algae (63.31%) than red (58.33%). Also, these two extracts effectively induced apoptosis and stopped the cell cycle in G0/G1 stage.

This study showed that the chloroform extract of these algae can be considered as a strong inhibitor for breast cancer.